My research revolves around understanding molecular mechanisms that control axon growth, with the ultimate goal of using this information to induce axon regeneration following injury. I have spent the last decade tackling the problem of why injured neurons in the central nervous system fail to upregulate the right sets of genes supportive of axon re-growth, which ultimately determines regenerative success. I have identified and addressed transcriptional and epigenetic barriers to re-growth using a combination of methods including Bioinformatics, Functional Genomics, and High-Content Screening. I am currently looking to transition to a tenure track position in India in the coming year.
PhD in Neuroscience, 2014
University of Wisconsin, Milwaukee
Bachelors in Biotechnology, 2009
Thanks to my current and previous funding sources!
Transcription factors (TFs) act as powerful levers to regulate neural physiology and can be targeted to improve cellular responses to injury or disease. Because TFs often depend on cooperative activity, a major challenge is to identify and deploy optimal sets. Here we developed a novel bioinformatics pipeline, centered on TF co-occupancy of regulatory DNA, and used it to predict factors that improve axon growth in corticospinal tract (CST) axons when combined with a known pro-regenerative TF, Klf6. Assays of neurite outgrowth confirmed cooperative activity by 12 candidates, and in vivo testing showed strong promotion of CST growth upon combined expression of Klf6 and Nr5a2. Transcriptional profiling of CST neurons identified Klf6/Nr5a2-responsive gene networks involved in macromolecule biosynthesis and DNA repair. These data identify novel TF combinations that promote enhanced CST growth, clarify the transcriptional correlates, and provide a bioinformatics roadmap to detect TF synergy. ### Competing Interest Statement The authors have declared no competing interest.